12 hours before collection (water is ok).
If you take biotin, wait 72 hours after your last dose before collecting.
Drink a glass of water. Electrolytes are fine (no sugar).
Warm water + soap for 20–30 seconds.

1–2 minutes of easy activity to warm hands/arms. Avoid hard workouts 24 hours prior.
Outer upper arm, flat area ~10 fingers below shoulder.

Shave excess hair; a clean surface improves the vacuum seal.
Activate the heat pack. Hold vertically on the site for 3 minutes; remove if too hot.
Use the alcohol pad; let the skin dry fully.
Pull off the cardboard safety tab covering the lever.
Unscrew cap → press tube into device with a small twist (tube lip faces back).
Peel off the adhesive liner using the back tab.
Press firmly on the warmed site with the tube pointing down. Adhesive maintains vacuum.


Press the button down till you hear a click. Flow usually starts in 30–90 seconds.
Remove when blood reaches the TOP FILL LINE or at 3 minutes — whichever comes first.
Twist-pull the tube off the device. If < 400 µL, use your backup kit and send both tubes.
Click the cap on; tap the tube and flip it 10× to mix (contents should be liquid, not clumpy).

Wipe with gauze, apply the bandage, and wash hands again.
Put the tube in the specimen bag. Fill in name/DOB/collection date card and place it in the pocket.
Add the frozen cold pack + specimen bag to the silver insulated pouch and seal.
Place the silver pouch into the kit box, then into the return mailer.
Set the package out and text a photo to your coordinator (or drop at an indoor FedEx, not a drop box).